Periodic Acid-Schiff (PAS) Stain
Intended Use:
This kit is for staining bone marrow and blood cellsmear.
Principle:
PAS Stain is based on the principle of periodic acid-Schiff reaction. Periodic acid can oxidize Ethylene Glycol
(-CHOH-CHOH) contained in glycogen polysaccharides of cells to turn into dialdehyde (-CHO-CHO).
Subsequently, aldehyde will react with colorless Fucshin to form a red-fuchsin compound incytoplasm.
Specifications:
|
Contents |
5Tests/Kit |
20Tests/Kit |
100Tests/Kit |
Components |
|
Fixative (solution A) |
1vial×5ml |
1vial×20ml |
1vial×100ml |
Ethanol |
|
Periodic Acid (solution B) |
1vial×5ml |
1vial×20ml |
1vial×100ml |
Periodic Acid |
|
Schiff's Reagent (solution C) |
1vial×5ml |
1vial×20ml |
1vial×100ml |
Fuchsin Basic |
|
Hematoxylin (solution D) |
1vial×5ml |
1vial×20ml |
1vial×100ml |
Hematoxylin |
Methods:
1. Dry the smear. Fix smear in solution A for 30 seconds and rinse with distilled water. Air dry or dry with
absorbent paper.
2. Drop solution B on slide to fully cover the smear for 5minutes. Rinse with distilled water and dry with filter
paper or dry in the air.
3. Drop solution C on slide to fully cover the smear for 10~15 minutes. Rinse with distilled water for 5 minutes.
4. Counterstain with solution D for 1~2 minutes. Rinsewith distilled water. Dry the smear for microscopic
examination.
