PAS Hepatin Stain
Intended use:
PAS Hepatin stain is intended for hepatin staining. Tissue is oxidized in periodic acid and color will develop after adding colorless Fucshin. This reaction is mainly used for examining polyose. For fungi and bacteria, the reaction also shows positive.
Principle:
Periodic acid is an oxidant which can destroy carbon bond of polyrose. First, tissues are oxidized in periodic acid solution to break open the carbon bond of glycol group or ammonia hydroxyl in tissue polyose, which results in the formation of aldehyde compound. Subsequently, the free aldehyde exposed will react with colorless Fucshin to form a new red- fuchsia compound.
Methods:
1. Deparaffinize sections to deionized water.
2. Rinse in distilled water for 1~2 minutes.
3. Oxidate sections in 0.5%Periodic acid for 10 minutes.
4. Rinse thoroughly in distilled water and dry sections with absorbent paper.
5. Stain in Schiff reagent (Colorless Fucshin solution) for 10~15 minutes.
6. Wash in running tap water for 10 minutes.
7. Stain nucleus with Hematoxylin for 1~2 minutes.
8. Dehydrate through 95%alcohol and 100%alcohol, clear in xylene.
9. Mount with mounting media.
Specifications
|
Contents |
3vialsx20ml/kit |
3Btlsx100ml/kit |
3Btlsx250ml/kit |
Components |
|
Schiff‘s Reagent |
20ml |
100ml |
250ml |
Alkaline Fucshin |
|
Periodic acid |
20ml |
100ml |
250ml |
Periodic acid |
|
Mayer Hematoxylin |
20ml |
100ml |
250ml |
Hematoxylin |
Precaution:
1. Store Colorless Fucshin solution in the refrigerator, and bring it to room temperature before use. After each use pour it back into the original bottle and store in the fridge. By following the above procedure, the Colorless Fucshin solution can be reused several times until rosiness appears.
2. The staining time in Colorless Fucshin solution will depend on the room temperature. In summer, 10 minutes is typically enough; in winter, however, it should be extended up to 20 minutes.
Expected Results:
|
Hepatin, neutral mucus |
—— |
Red |
|
Nuclei |
—— |
Blue |