Phosphotungstic Acid Hematoxylin Stain

Principle:

Mature hematin reacts with tungsten to form blue sediment which strongly combines with tissue. Reddish brown portion is stained by Phosphotungstic Acid.

 

Specifications:

Contents	1 Btl ×100ml/Kit	1Btl×250ml/Kit	Components
Phosphotungstic  Acid Hematoxylin Stain	100ml	250ml	Haematoxylin, Phosphotungstic Acid

 

 

 

 

Methods:

1. Put tissue into xylene. In subsequent step, put the tissue in tap water.

2. Add distilled water into 5% iron alum and mordant for 30 minutes.

3. Rinse with distilled water after washing in tap water.

4. Oxidize the smear in 2.5% potassium permanganate and rinse with tap water.

5. Place in 2.5% oxalate till the smear turns white.

6. Rinse in tap water, distilled water. Then, dry with absorbent paper.

7. Place in PTAH stain for 12~24hours, or incubate for an hour.

8. Differentiate in 95% alcohol for 3~5 seconds.(As soon as possible)

Rapidly dehydrate; clear and mount.

 

Expected Results:

Rhabdium, Glia, Fibrin, Nuclei……..……..Blue

Collagen fibers……………………………..Reddish Brown

 

Precaution:

1. Do not rinse with water after staining with Phosphotungstic Acid Haematoxylin. Quickly rinse in 95% alcohol, because alcohol or water will wash red color away.

2. Do not overstain the smear, since Phosphotungstic Acid Haematoxylin is progressive stainning reagent. Examine under a microscope in certain period.

3. The procedure of staining is to be operated in a staining jar.